ISBN: 3-540-61233-5
TITLE: Proteolytic Enzymes
AUTHOR: Sterchi, Erwin E.; Stcker, Walter (Eds.)
TOC:

Chapter 1
Introduction: Nomenclature and Classes of Peptidases
A.J. KENNY 1
Chapter 2
Chromogenic Peptide Substrates
H. KIRSCHKE AND B. WIEDERANDERS 11
Chapter 3
Fluorometric Assays
DAVID S. AULD 30
Chapter 4
Detection of Proteolytic Enzymes Using Protein Substrates
KEVIN K.W. WANG 49
Chapter 5
Zymography, Casein Zymography, and Reverse Zymography: Activity assays for proteases and their inhibitors
GARY W. OLIVER, WILLIAM G. STETLER-STEVENSON
AND DAVID E. KLEINER 63
Chapter 6
Genetic - Based Assays of Viral Proteases
C. RIZZO, Y-S. E. CHENG AND B. KORANT 77
Chapter 7
Strategies for Inhibiting Proteases of Unknown Mechanism
RUSSEL L. WOLZ 90
Chapter 8
Purification of Proteases
NIGEL M. HOOPER 109
Chapter 9
Crystallization of Proteinases
MARGIT M.T. BAUER AND MILTON T. STUBBS 124
Chapter 10
Basis Kinetic Mechanisms of Proteolytic Enzymes
L. POLGR 148
Chapter 11
Kinetic Analysis of Protease Inhibition by Synthetic Inhibitors
C. GRAHAM KNIGHT 167
Chapter 12
Phosphinic Peptide Libraries for Proteolytic Enzymes
V. DIVE, J. JIRACEK, A. YIOTAKIS 188
Chapter 13
Functional Expression of Recombinant Proteases
DIETER BRMME AND BRIAN F. SCHMIDT 199
Chapter 14
Proteases in Peptide Mapping and Sequencing
JOSEF KELLERMANN 233
Chapter 15
Limited Proteolysis in the Study of Protein Conformation
ANGELO FONTANA, PATRIZIA POLVERINO DE LAURETO, VINCENZO DE FILIPPIS, ELENA SCARAMELLA AND MARCELLO ZAMBONIN 253
Chapter 16
Limited Proteolysis in the Study of Membrane Proteins
HASSAN Y. NAIM 281
Chapter 17
Segregating Cells - Proteases in Tissue Culture
U.N. WIESMANN 298
Chapter 18
Protease-Catalyzed Peptide Synthesis
D. ULLMANN AND H.-D. JAKUBKE 312
Appendix
Supplement to Chapter 9: Crystallization Conditions for Proteases
M.T. STUBBS AND M.M.T. BAUER 331
Subject Index 333
END
