ISBN: 3-540-65149-7
TITLE: In Living Color
AUTHOR: Diamond, Rochelle A.; DeMaggio, Susan (Eds.)
TOC:

Section 1 
A Palette of Living Colors 
ROCHELLE DIAMOND 3 
The Colorful History of Flow Cytometry 
SUSAN DEMAGGIO 9 
Section 2 
Getting Started 
ROCHELLE DIAMOND 13 
Chapter 1 
How the FACSCalibur and FACS Vantage Work 
and Why It Matters 
TOM FREY 15 
Chapter 2 
How Flow Cytometers Work - and Don't Work 
HOWARD SHAPIRO 39 
Chapter 3 
Flow Cytometry Standard (FCS) Data File Format 
LARRY SEAMER 57 
Chapter 4 
Options for Data Display 
SUSAN DEMAGGIO AND ROCHELLE DIAMOND 62 
Chapter 5 
Guidelines to Improve Flow Cytometry Data Display 
and Interpretation 
WENDY SCHOBER-DITMORE AND DOROTHY LEWIS 71 
Chapter 6 
Data Management and Storage 
DAVID M. CODER 76 
Chapter 7 
Coloring up: A Guide to Spectral Compensation 
ROCHELLE A. DIAMOND 92 
Chapter 8 
Quality Control Guidelines for Research Flow Cytometry 
ROCHELLE A. DIAMOND 98 
Section 3 
Sample Preparation and Cell Surface Staining 
ROCHELLE A. DIAMOND 109 
Chapter 1 
Cell Preparation and Enrichment for FCM Analysis 
and Cell Sorting 
ROCHELLE A. DIAMOND, HUA WANG, FEI CHEN, 
AND MINDY WILKE-DOUGLAS 111 
Chapter 2 
The Basics of Staining for Cell Surface Proteins 
ALICE L. GIVANS 142 
Chapter 3 
Phenotypic Analysis Using Five-Colour 
Immunofluorescence and Flow Cytometry 
ANDY BEAVIS 165 
Chapter 4 
Detection of Cell Surface Antigens Using Antibody- 
Conjugated Fluorospheres and Flow Cytometry 
ANDY BEAVIS 175 
Chapter 5 
Gel Microdrop Encapsulation for the Frugal Investigator 
DAVID C. MCFARLAND AND GARY DURACK 184 
Section 4 
Reporter Genes and Cell Trackers 
ROCHELLE A. DIAMOND 197 
Chapter 1 
Flow Cytometric Analysis of GFP Expression 
in Mammalian Cells 
STEVEN R. KAIN 199 
Chapter 2 
FACS-Gal: Flow Cytometric Assay of beta-galactosidase 
in Viable Cells 
STEVEN FIERING 227 
Chapter 3 
Utilization of a Bicistronic Expression Vector for Analysis 
of Cell Cycle Kinetics of Cytotoxic and Growth-arrest Genes 
NICHOLAS E. POULOS, ERIC STANBRIDGE, 
AND SUSAN DEMAGGIO 259 
Chapter 4 
NGFR as a Selectable Cell Surface Reporter 
G. RAGHU, M. MUNN, S. W. PARK, C-H PAN, AND A. DAYN 267 
Chapter 5 
Assessment of Cell Migration in vivo Using the 
Fluorescent Tracking Dye PKH26 and Flow Cytometry 
ANDY BEAVIS 276 
Chapter 6 
In vivo Biotinylation for Cell Tracking 
and Survival/Lifespan Measurements 
KENNETH A. AULT AND CATHY KNOWLES 284 
Chapter 7 
Enrichment and Selection for Reporter Gene 
Expression with MACSelect 
MINDY WILKE-DOUGLAS AND UTE BEHRENS-JUNG 291 
Chapter 8 
Use of PKH Membrane Intercalating Dyes to Monitor 
Cell Trafficking and Function 
REBECCA POON, BETSY M. OHLSSON-WILHELM, 
BRUCE BAGWELL, AND KATHARINE A. MUIRHEAD 302 
Section 5 
Flow Cytometry of Nucleic Acids 
ROCHELLE A. DIAMOND 355 
Chapter 1 
RNA Content Determination Using Pyronin Y 
HOWARD M. SHAPIRO 357 
Chapter 2 
DNA Content Determination 
DEREK DAVIES AND CLARE HUGHES 361 
Chapter 3 
Dead Cell Discrimination 
DEREK DAVIES AND CLARE HUGHES 372 
Chapter 4 
Detecting Apoptosis 
DEREK DAVIES AND INGRID SCHMID 377 
Chapter 5 
Chromosome Preparation - Polyamine Method 
DEREK DAVIES AND CLARE HUGHES 385 
Chapter 6 
"D-Flowering" - The Flow Cytometry of Plant DNA 
JOHN MOTLEY, K. YOKOYA, AND A.V. ROBERTS 391 
Chapter 7 
Flow Cytometry Analysis of Marine Picoplankton 
DOMINIQUE MARIE, N. SIMON, L. GUILLON, 
F. PARTENSKY, AND D. VAULOT 421 
Chapter 8 
Yeast DNA Flow Cytometry 
WENDY SCHOBER-DITMORE AND JEFF BACHANT 455 
Section 6 
Intracellular Physiological and Antibody Probes 
ROCHELLE A. DIAMOND 461 
Chapter 1 
Calcium Flux Protocol: INDO-1 Stained Thymocytes 
GEOFFREY W. OSBORNE AND PAUL WARING 463 
Chapter 2 
Intracellular pH Measured by ADB 
MICHAEL FOX 472 
Chapter 3 
Measuring Intracellular pH Using SNARF-1 
MICHAEL FOX 481 
Chapter 4 
FACS Analysis of Ph and Apoptosis 
GRANT W. MEISENHOLDER, JUDY NORBERG, 
AND ROBERTA A. GOTTLIEB 490 
Chapter 5 
Measurement of Ligand Acidification Kinetics for 
Adherent and Non-Adherent Cells 
SHEREE LYNN RYBACK AND ROBERT F. MURPHY 496 
Chapter 6 
Intracellular Antigen Detection by Flow Cytometry 
INGRID SCHMID 524 
Chapter 7 
Flow Cytometric Measurement of Nuclear Matrix Proteins 
JOSEPH R. DYNLACHT 532 
Chapter 8 
CellProbe Flow Cytoenzymology 
SUSAN DEMAGGIO 539 
Chapter 9 
Intracellular Cytokine Detection 
MARY A. YUI 552 
Section 7 
Electronic Cell Sorting 
ROCHELLE A. DIAMOND 563 
Chapter 1 
Creating Standard and Reproducible Sorting Conditions 
MARTY BIGOS, RICHARD T. STOVEL, AND DAVID R. PARKS 565 
Chapter 2 
Sterilization for Sorting 
STEVEN MERLIN 572 
Chapter 3 
High Speed Cell Sorting Using the Cytomation CICERO
and MoFlo Systems 
PETER LOPEZ 577 
Chapter 4 
Purification of Mouse Fetal Liver Hematopoietic Stem Cells 
SEAN J. MORRISON 585 
Chapter 5 
Methods for Preparing Sorted Cells as Monolayer 
Specimens 
ROBERT C. LEIF 592 
Chapter 6 
Fluorescent in Situ Hybridization Analysis on 
Cells Sorted onto Slides 
KAREN CHEW AND ROBERT C. LEIF 620 
Section 8 
Core Facilities 
ROCHELLE A. DIAMOND 633 
Chapter 1 
Core Facility Management 
STEVEN MERLIN 635 
Chapter 2 
Funding for Core Facilities 
LARRY A. SKLAR 642 
Chapter 3 
Biosafety in the Flow Cytometry Laboratory 
INGRID SCHMID 655 
Section 9 
Unlocking the Spectrum of Possibilities 
ROCHELLE A. DIAMOND 669 
Appendices 
GFP 
STEVEN KAIN 677 
Fluorescent Protein Newsgroup 
STEVEN KAIN 679 
Anderson's Timesaving Comparative Guides 681 
ARC Lamp Flow Cytometers 
JEFF HARVEY 683 
CompCyte Laser Scanning Cytometer 
SUSAN DEMAGGIO 691 
Coulter Flow Cytometry Systems 
SUSAN DEMAGGIO 697 
Cytomation Products for Upgrading Flow Cytometers 
SUSAN DEMAGGIO 701 
Glossary 703 
Lasers in Flow Cytometry 
PAUL GINOUVES 715 
Resources for Reagents 731 
Flow Cytometry Internet Access 
and World Wide Web Sites of Interest 732 
User Groups Around the Country 736 
Commercial Suppliers for Flow Related Products 739 
Data Analysis for Cell Census Plus(TM) System 
REBECCA POON 754 
Monoclonal Antibody Production 
GARY WOO 776 
Subject Index 789 
END
