ISBN: 3-540-64601-9
TITLE: Nonradioactive Analysis of Biomolecules
AUTHOR: Kessler, Christoph (Ed.)
TOC:

Introduction 
Chapter 1 
General Aspects of Nonradioactive Analysis of Biomolecules 
CHRISTOPH KESSLER 1 
Part A Standard Nonradioactive Labeling Systems 
Chapter 2 
Overview of Nonradioactive Labeling Systems 
CHRISTOPH KESSLER 41 
The DIG (Digoxigenin:Anti-Digoxigenin) System 
Chapter 3 
Overview on the Digoxigenin:Anti-Digoxigenin (DIG) System 
CHRISTOPH KESSLER 59 
Chapter 4 
Labeling and Detection of Nucleic Acids 
HANS-JOACHIM HLTKE, KELLY KING, CATHY HOOVER, 
IRMGARD OBERMAIER, BARBARA RGER, KLAUS MHLEGGER, 
AND CHRISTOPH KESSLER 61 
Chapter 5 
Labeling and Detection of Proteins and Glycoproteins 
ANTON HASELBECK AND WOLFGANG HSEL 94 
The Biotin (Biotin: Streptavidin) System 
Chapter 6 
Labeling and Detection of Nucleic Acids 
AYOUB RASHTICHIAN AND JESSE MACKEY 111 
Chapter 7 
Labeling of Proteins During In Vitro Translation 
THOMAS METZLER AND HANS-JOACHIM HOELTKE 137 
Chapter 8 
Labeling and Detection of Proteins and Glycoproteins 
EDWARD A. BAYER AND MEIR WILCHEK 155 
5-BrdU, AAF, Gold Labeling 
Chapter 9 
In Vivo Labeling of DNA Probes with 5-BrdU 
JEAN-LUC GUESDON 168 
Chapter 10 
AAF-labeling of DNA and Oligonucleotides 
JEAN-LUC GUESDON, JACQUES AURIOLA, AND DANILE CHEVRIER 180 
Chapter 11 
Colloidal Gold as a Marker in Molecular Biology: 
The Use of Ultra-Small Gold Conjugates 
PETER F. E. M. VAN DE PLAS AND JAN L. M. LEUNISSEN 193 
Direct Enzyme Label 
Chapter 12 
Direct Peroxidase Labeling of Hybridization Probes 
and Chemiluminescence Detection 
IAN DURRANT 206 
Chapter 13 
The SNAP System 
JAMES E. MARICH AND JERRY L. RUTH 216 
Part B Specialized Nonradioactive Detection Systems 
Chapter 14 
Overview on Nonradioactive Detections Systems 
CHRISTOPH KESSLER 227 
Chapter 15 
Overview of Colorimetric, Chemiluminometric, 
and Fluorimetric Detection Systems 
HANS-JOACHIM GUDER, DIETER HEINDL, AND HANS-PETER JOSEL 240 
Colorimetric Systems 
Chapter 16 
Indigo/Tetrazolium Dyes 
HANS-JOACHIM GUDER, DIETER HEINDL, AND HANS-PETER JOSEL 249 
Chapter 17 
Azo Dyes 
PETER SCHSSLER, CHRISTOPH G. GREVELDING, 
AND WERNER KUNZ 253 
Luminescence Systems 
Chapter 18 
Chemiluminescent Detection with Horseradish Peroxidase 
and Luminol 
DIETER HEINDL, HANS-PETER JOSEL 258 
Chapter 19 
Chemiluminescence: 
Properties of 1,2-Dioxetane Chemiluminescence 
IRENA BRONSTEIN AND LARRY KRICKA 262 
Chapter 20 
Electrochemiluminescence: Ruthenium Complexes 
JOHN H. KENTEN 271 
Chapter 21 
Bioluminescence: D-Luciferin as Substrate 
DUSICA GABRIJELCIC-GEIGER, WERNER MISKA, 
AND REINHARD ERICH GEIGER 276 
Fluorescence Systems 
Chapter 22 
Labeling of Biomolecules with Fluorophores 
DIETER HEINDL, HANS-PETER JOSEL 284 
Chapter 23 
Time-Resolved Fluorescence 
ELEPHTERIOS P. DIAMANDIS AND THEODORE K. CHRISTOPOULOS 289 
Part C Amplification Systems for Sensitivity Enhancement 
Chapter 24 
Overview of Amplification Systems 
CHRISTOPH KESSLER 297 
PCR Target Amplification 
Chapter 25 
Amplification of Nucleic Acids by Polymerase Chain Reaction: 
Overview on Basic Principles 
ARNDT ROLFS, ULRICH FINCKH, AND PETER BAUER 311 
Chapter 26 
PCR: Overview on Application Formats in Research 
and Clinical Diagnosis 
GERD HABERHAUSEN 327 
Chapter 27 
PCR Amplification for the Generation of DIG-Labeled Probes 
BARBARA RGER AND RDIGER RGER 335 
Chapter 28 
In Situ PCR Amplification of cDNA 
GERARD J. NUOVO 343 
Strand Displacement Amplification (SDA) Target Amplification 
Chapter 29 
SDA Target Amplification 
CATHERINE A. SPARGO, CHERYL H. DEAN, COLLEEN M. NYCZ, 
AND G. TERRANCE WALKER 356 
Chapter 30 
In Situ Strand Displacement Amplification 
ROBERT A. REID AND G. TERRANCE WALKER 367 
Transcription-Mediated Amplification (TMA) Target Amplification 
Chapter 31 
Isothermal Amplification of RNA 
by Transcription-Mediated Amplification (TMA) 
STEVEN T. BRENTANO, AND SHERROL H. MCDONOUGH 374 
Ligase Chain Reaction (LCR) Amplification 
Chapter 32 
Ligase Chain Reaction 
GEORG H. SHIMER AND KEITH BACKMAN 381 
Signal Amplification 
Chapter 33 
Branched DNA (bDNA) Technology 
MICKEY URDEA AND LINDA WUESTEHUBE 388 
Chapter 34 
rDNA Amplification: Application of 16S rDNA-Based Methods 
for Bacterial Identification 
FREDERICK A. RAINEY AND ERKO STACKEBRANDT 396 
Chapter 35 
PRINS: Primed In Situ Labeling and Hybridization in one Step 
JORN KOCH 407 
Part D Application Formats 
Chapter 36 
Overview on Amplification Formats 
CHRISTOPH KESSLER 419 
Chapter 37 
Overview on Factors Influencing Nucleic Acid Hybridization 
CHRISTOPH KESSLER 437 
Blot Formats: Nucleic Acids 
Chapter 38 
Dot, Southern, and Northern Blots 
BARBARA RGER 443 
Chapter 39 
PNA Pre-Gel Hybridization - An Alternative to Southern Blotting 
MICHAEL EGHOLM AND HEATHER PERRY-O'KEEFE 464 
Chapter 40 
Multilocus DNA Fingerprinting Using Nonradioactively Labeled 
Oligonucleotide Probes Specific for Simple Repeat Elements 
JRG T. EPPLEN AND JUDITH MTH 468 
Blot Formats: Proteins and Glycoproteins 
Chapter 41 
Detection of Proteins and Glycoproteins on Western Blots 
ANTON HASELBECK AND WOLFGANG HSEL 475 
In Situ Formats 
Chapter 42 
PNA as Specific Probe for In Situ Hybridization 
to Metaphase Chromosomes 
BODIL LOMHOLT, SUNE FREDERIKSEN, AND PETER E. NIELSEN 478 
Chapter 43 
A Highly Sensitive Method for Detecting Peroxidase in In Situ 
Hybridization or Immunohistochemical Assays 
JAMES G. LAZAR AND FLOYD E. TAUB 484 
Chapter 44 
Non-Fluorescent Differentiation of Viral 
and Chromosomal Nucleic Acids in Individual Nuclei 
SHIRLEY A. SOUTHERN AND C. SIMON HERRINGTON 494 
Chapter 45 
Virus Detection in Biopsy Specimens 
VEIJO HUKKANEN AND PIRKKO HEINO 505 
Chapter 46 
Mapping of Polytene Chromosomes 
CHRISTIANE KRAEMER AND ERWIN R. SCHMIDT 516 
Chapter 47 
Fluorescence In Situ Hybridization on Banded Chromosomes 
N. ARNOLD, M.B. UPENDER, T. RIED, J. WIENBERG, AND D. C. WARD 524 
Chapter 48 
Spectral Karyotyping: New Tools for Multicolor Chromosome Analysis 
IRIT BAR-AM, MEENA AUGUSTUS, GEORGE MACNAMARA, 
THOMAS RIED, AND EVELIN SCHRCK 539 
Chapter 49 
Chromosome Analysis by Multiplex-FISH (M-FISH) 
MICHAEL SPEICHER 556 
Chapter 50 
Whole Mount In Situ Hybridization for the Detection 
of mRNA in Drosophila Embryos 
DIETHARD TAUTZ 573 
Chapter 51 
Double Labeling of mRNA and Proteins in Drosophila Embryos 
BARBARY COHEN AND STEPHAN M. COHEN 581 
Quantitative Formats 
Chapter 52 
Detection of DNA/RNA Target/Probe Complexes 
with DNA/RNA-Specific Antibodies 
FRANCOIS COUTLE, ROBERT H. YOLKEN, AND RAPHAEL P. VISCIDI 594 
Chapter 53 
Molecular Beacons: Hybridization Probes for Detection of Nucleic 
Acids in Homogeneous Solutions 
SANJAY TYAGI, SALVATORE A. E. MARRAS, JACQUELINE A. M. VET, 
AND FRED RUSSELL KRAMER 606 
Chapter 54 
Detection of DIG-Labeled Amplicons 
on Streptavidin-Coated Microtiter Plates 
STEFANIE KHLER, VOLKER SCHLTER, AND RUDOLF SEIBL 617 
Chapter 55 
A Fluorogenic PCR-Based Assay for the Rapid Detection of Salmonella 
CHRISTINE PASZKO-KOLVA AND CARL YAMASHIRO 622 
Chapter 56 
PCR-Coupled Activation of Intercalating Dyes 
TAKAHIKO ISHIGURO 631 
Chapter 57 
Simultaneous SDA and Fluorescence Polarization Detection 
PATRICIA A. SPEARS AND G. TERRANCE WALKER 640 
Sequencing Formats 
Chapter 58 
Large-Scale Genomic Sequencing Using Four Color Fluorescent 
Detection - Principles and Protocols 
MATTHIAS PLATZER, GERALD NYAKATURA, 
AND ANDR ROSENTHAL 648 
Chapter 59 
DIG DNA Sequencing with Chemiluminescent or Dye Substrates 
GREGOR SAGNER 675 
Chapter 60 
DNA Sequencing: Chemiluminescent Detection 
with the 1,2 Dioxane CSPD 
CHRIS S. MARTIN AND IRENA BRONSTEIN 689 
Chapter 61 
Direct-Blotting-Electrophoresis (DBE) for DNA Sequencing 
THOMAS M. POHL 695 
Chapter 62 
Sequence Analysis of Nucleic Acids by Mass Spectrometry 
DIRK VAN DEN BOOM, CHRISTIAN JURINKE, DONG-JING FU, 
AND HUBERT KSTER 701 
Array Formats 
Chapter 63 
Mismatch Analysis by PNA Arrays 
ANE LESTER, ALBERT GEIGER, JRG KLEIBER, AND HENRIK ORUM 713 
Chapter 64 
Oligonucleotide Arrays for the Detection of ras Mutations 
ROBERT S. MATSON 724 
Chapter 65 
Use of Oligonucleotide Arrays for Gene Expression Monitoring 
MICHAEL C. BYRNE AND MARYANN Z. WHITLEY 738 
Subject Index 745 
Appendix: Reference List for Nonradioactive Digoxigenin (DIG) System 
END
