ISBN: 3-540-65630-8
TITLE: Flow Cytometry and Cell Sorting
AUTHOR: Radbruch, Andreas (Ed.)
TOC:

Part I Flow Cytometry 1
Chapter 1 Operation of a Flow Cytometer
Christoph Gttlinger, Birgit Mechtold and Andreas Radbruch 3
Part II Immunofluorescence 27
Chapter 2 Conjugation of Fluorochromes, Haptens, and Phycobiliproteins to Antibodies
Werner Mller 29
Chapter 3 Immunofluorescence: Basic Considerations
Andreas Radbruch 38
Chapter 4 Multicolor Immunofluorescence Analysis
Chia-Huei Chen, Kerstin Willmann, Dieter Recktenwald 53
Chapter 5 Combined Intracellular and Surface Staining: Immunofluorescence of Cytokines in T Cells
Mario Assenmacher 63
Chapter 6 Cytometry and Sorting of Live Cells According to Their Secreted Products
Rudolf A. Manz 71
Chapter 7 Cytometry of Rare Surface Molecules by Magnetofluorescent Liposomes
Alexander Scheffold 77
Chapter 8 Scatchard Analysis by Flow Cytometry
Robert F. Murphy 82
Chapter 9 Microbead Assay for Quantification of Neuronal Adhesion Molecule Interaction by Flow Cytometry
Attila Trnok, Ursel Nhrenberg, Hans-Jrgen Vollmer and Stephan Schuhmacher 86
Part III DNA and Proliferation 99
Chapter 10 Preparation and Staining of Fixed Cells for High-Resolution DNA Analysis
Friedrich F. Otto 101
Chapter 11 Simultaneous Flow Cytometric Detection of Bromodeoxyuridine Incorporation and Cell Surface Marker Expression
Werner Mller 105
Chapter 12 High-Resolution Cell Cycle Analysis: The Flow Cytometric Bromodeoxyuridine-Hoechst Quenching Technique
Manfred Kubbies 112
Part IV Cellular Activation and Biochemistry 125
Chapter 13 Cell Activation: Indo-1 Ratiometric Analysis of Intracellular Ionized Calcium
Manfred Kubbies 127
Chapter 14 Detection and Purification of Rare Responders by Fixed-Time Flow Cytometry
Attila Trnok and Henning Ulrich 140
Chapter 15 Biochemical Parameters of Cells Function
Gregor Rothe and Gnther Valet 159
Chapter 16 The FACS-Gal Assay
Steffen Jung 183
Chapter 17 Ligand Acidification by Nonadherent Cells
Robert F. Murphy 186
Part V Cell Sorting 195
Chapter 18 Powerful Preselection
Charlotte Esser 197
Chapter 19 Magnetic Selection of Transfected Cells
Gregor Siebenkotten and Ute Behrens-Jung 210
Chapter 20 High Gradient Magnetic Cells Sorting
Stefan Miltenyi and Jrgen Schmitz 218
Chapter 21 Setup of a Flow Sorter
Christoph Gttlinger, Birgit Mechtold, Klaus Meyer and Andreas Radbruch 248
Chapter 22 Sorting of Rare Cells
Walter Weichel, Sigrid Irlenbusch, Kimitaka Kato and Andreas Radbruch 255
Chapter 23 Fluorescence-Activated Chromosome Sorting
Judith A. Fantes and Daryll K. Green 266
Chapter 24 Analysis and Sorting of Plant Chromosomes by Flow Cytometry
Jacky Veuskens, Dominique Marie, Stefan Hinnisdaels and Spencer C. Brown 277
Chapter 25 Large Particle Sorting
David W. Galbraith and Sergio Lucretti 293
Part VI Safety 319
Chapter 26 Biological and Laser Safety
Klaus L. Meyer 321
Appendices
Birgit Mechtold and Andreas Radbruch 327
Glossary 329
Suppliers 331
Cytometry on the Internet 347
Subject Index 349
END
