ISBN: 4-431-70231-8
TITLE: Molecular Histochemical Techniques
AUTHOR: Koji, Takehiko (Ed.)
TOC:

Part I
Chapter 1
Introduction to the Detection of DNA Strand Breaks
T. KOJI 3
Chapter 2
In Situ Nick Translation
S. HASHIMOTO, T. KANEMATSU 13
Chapter 3
In Situ Nick End-Labeling: Light Microscopical
S. OBA, T. KOJI 19
Chapter 4
In Situ Nick End-Labeling: Electron Microscopical
Y. OTSUKI, Y. ITO 31
Part II
Chapter 5
Introduction to the Detection of Specific DNA and RNA Sequences
T. KOJI 49
Chapter 6
In Situ Hybridization for DNA: Fluorescent Probe
M. MATSUTA, M. MATSUTA 65
Subprotocol 1: Fluorescence In Situ Hybridization (FISH) 68
Subprotocol 2: Protocol for Rapid Procedure 88
Subprotocol 3: Protocol for Codenaturation 90
Subprotocol 4: Comparative Genomic Hybridization (CGH) 92
Chapter 7
In Situ Hybridization for RNA: Radioactive DNA Probe
T. SAWAI, M. UZUKI 100
Chapter 8
In Situ Hybridization for RNA: Radioactive RNA Probe - A Protocol for Practice With a Kit
H. YOSHIOKA, S. NOJI 115
Chapter 9
In Situ Hybridization for RNA: Nonradioactive probe: ds cDNA Probe
H. MATSUMI, T. YANO 128
Chapter 10
In Situ Hybridization for RNA: Nonradioactive Probe: ss cDNA Probe
Y. KANEMITSU, T. KOJI 139
Chapter 11
In Situ Hybridization for RNA: Nonradioactive Probe: Oligo-DNA Probe: T-T Dimer
Y. HISHIKAWA, N. NAGASUE, M. MURAKAMI, M. KASAHARA 154
Chapter 12
In Situ Hybridization for RNA: Nonradioactive Probe: Oligo-DNA Probe: Digoxigenin (I)
Y. IWASAKI, I. KATO 172
Chapter 13
In Situ Hybridization for RNA: Nonradioactive Probe: Oligo-DNA Probe: Digoxigenin (II)
M. MIYAZAKI, Y. OZONO, T. HARADA, S. KOHNO 182
Chapter 14
In Situ Hybridization for RNA: Nonradioactive Probe: RNA Probe
H. OHTANI 196
Chapter 15
Electron Microscopic in Situ Hybridization and its Combination With Immunohistochemistry
A. MATSUNO, T. NAGASHIMA, R. Y. OSAMURA, K. WATANABE 204
Chapter 16
Whole Mount in Situ Hybridization for mRNA detection in Chick Embryos
H. OHUCHI, S. NOJI 222
Part III
Chapter 17
Introduction to the Detection of Transcription Regulatory Proteins
T. KOJI 237
Chapter 18
Southwestern Histochemistry
K. KOMUTA, T. KANEMATSU, T. KOJI 244
Subject Index 253
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