## Chapter 9/Figure 4.jpg Back | Home | Next | Differential density scatter plots and iso-relational surface mapping. (A) and (B) represent the spatial distribution of cholinergic (dots in A) and parvalbumin (dots in B) cells from the same brain showed separately. Filled circles mark the high density locations where the density of cholinergic or parvalbumin cells is higher than 15 cells in the unit space (250 x 250 x 50 microns). (C) The scatter plots of both cholinergic (red in the color version of this figure) and parvalbumin (green) cells are superimposed on the iso-relational surface (dark solid area; violet in the CD-ROM file) where the density of both the cholinergic and parvalbumin cells is > 5 and the ratio of cholinergic/parvalbumin cells is at least 0.5 or higher. (D) Merging the cholinergic/parvalbumin, cholinergic/calbindin and cholinergic/calretinin iso-relational surfaces (using cell density greater than 5 in the unit space) into one scheme reveals that the cholinergic 'column' can be parcellated into clusters of different sizes. Different shading of surfaces cover the spaces where the relationship of cholinergic cells to parvalbumin (green in the color version), calretinin (yellow) and calbindin (blue) neurons is similar. (0.5 or higher).