Chapter 9/Figure 4.jpg
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Differential density scatter plots and iso-relational surface mapping. (A)
and (B) represent the spatial distribution of cholinergic (dots in A) and
parvalbumin (dots in B) cells from the same brain showed separately. Filled
circles mark the high density locations where the density of cholinergic
or parvalbumin cells is higher than 15 cells in the unit space (250 x 250
x 50 microns). (C) The scatter plots of both cholinergic (red in the color
version of this figure) and parvalbumin (green) cells are superimposed on
the iso-relational surface (dark solid area; violet in the CD-ROM file)
where the density of both the cholinergic and parvalbumin cells is > 5 and
the ratio of cholinergic/parvalbumin cells is at least 0.5 or higher. (D)
Merging the cholinergic/parvalbumin, cholinergic/calbindin and cholinergic/calretinin
iso-relational surfaces (using cell density greater than 5 in the unit space)
into one scheme reveals that the cholinergic 'column' can be parcellated
into clusters of different sizes. Different shading of surfaces cover the
spaces where the relationship of cholinergic cells to parvalbumin (green
in the color version), calretinin (yellow) and calbindin (blue) neurons
is similar. (0.5 or higher). |