Chapter 17/Figure 6.JPG
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Comparison of conventional histological staining to MRM imaging for the
detection of 6-hydroxy dopamine induced neurotoxicity in rat brain. A section
has been selected which demonstrates the damage caused by the treatment
with the toxin. Sections were cut from this region of the brain. The conventional
Nissl stained section from this region does not show significant differences
at this level of magnification (A). A second section from this region has
been stained with tyrosine hydroxylase, an established marker for dopaminergic
neurons. As can be seen, there is a significant depletion of staining in
the substantia nigra in the treated hemisphere (B). The MRM section has
been pseudocolored in order to visualize subtle contrast differences (C).
The left and right hemispheres have diffuse regions of contrast differences
indicating that the unilateral treatment has significant effects. |
