Chapter 17/Figure 7.jpg
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Three-dimensional
representation of mid-infrared microspectroscopic dataset for a mouse cerebellum.
A mid-infrared spectral image has collected for a 10 micron thick section
of a mouse cerebellum. The third dimension is the spectroscopic data. This
can be visualized as a stack (left). The contrast can be adjusted to highlight
special structural features of the section (center). The lower threshold
has been increased above the level of "noise". Through contrast enhancement,
spectral images for the protein and lipid fractions can be identified, highlighted
and identified demonstrating the differential distribution of lipid and
protein component gradients (right). The protein absorbance wavenumber is
highlighted in red and the lipid absorbance wavenumber in green. |
